研究実績

2017年

Shibata K, Koyama T, Inde S, Iwai S, Chaen S

Mutations in the SH1 helix alter  the thermal properties of myosin II.

Biophysics and Physicobiology  (2017)  14: 67-73

 

2016年

Sugi H and Chaen S

Force-Velocity Relation:Its Implication about Molecular Mechanism of Muscle Contraction

Journal of Nanomedicine & Nanotechnology (2016) 7 : 5 DOI:10.4172/2157-7439.1000401

 

2016年

Haruo Sugi, Maki Yamaguchi, Tetsuo Ohno, Takakazu Kobayashi, Shigeru Chaen, &

Hiroshi Okuyama

Tension Recovery following Ramp-Shaped Release in High-Ca and Low-Ca Muscle Fibers:

Evidence for the Dynamic State of AMADP Myosin Heads in the Absence of ATP.

PLOS ONE| DOI:10.1371/journal.pone.0162003 September 1. 2016

 

2016年

Haruo Sugi & Shigeru Chaen

Evidence against the Swinging Lever Arm Mechanism in Muscle Contraction Based on

the Effect ofAntibodies to Myosin Head.

J Nanomed Nanotechnol 7: 377. doi:10.4172/2157-7439.1000377

 

2016年

Takahiro Kobatake, Kento Akatsuka, Miku Nemoto & Shigeru Chaen

Relation between the molecular weight size of cargo and the nuclear transport time.

Proceedings of the Institute of Natural Sciences Nihon University 51, 325-331 (2016)

 

2015年

Ryosuke Yamagishi, Takahide Okuyama, Shuntaro Oba, Jiro Shimada, Shigeru Chaen, Hiroki Kaneko
Comprehensive analysis of the dynamic structure of nuclear localization signals
Biochemistry and Biophysics Reports 4, (2015) 392-396

 

2015年

Haruo Sugi, Shigeru Chaen, Tsuyoshi Akimoto, Hiroki Minoda, Takuya Miyakawa, Yumiko Miyauchi, Masaru Tanokura, Seiryo Sugiura

Electron microscopic recording of myosin head power stroke in hydrated myosin filaments.

Scientific Reports 5, 15700 (2015) DOi: 10.1038/srep15700

 

2014年

Haruo Sugi, Shigeru Chaen, Takakazu Kobayashi, Takahiro Abe, Kazushige Kimura, Yasutake Saeki, Yoshiki Ohnuki, Takuya Miyakawa, Masaru Tanokura, Seiryo Sugiura

Definite Differences between In Vitro Actin-Myosin Sliding and Muscle Contraction as Revealed Using Antibodies to Myosin Head.

PLos One. 2014 9(6): e93272

 

2013年

Haruo Sugi, Takahiro Abe, Takakazu Kobayashi, Shigeru Chaen, Yoshiki Ohnuki, Yasutake Saeki, Seiryo Sugiura Enhancement of Force Generated by Individual Myosin Heads in Skinned Rabbit Psoas Muscle Fibers at Low Ionic Strength”,

PLoS One. 2013 May 15: 8(5): e63658

 

2013年

Takahiro Maruta, Takahiro Kobatake, Hiroyuki Okubo and Shigeru Chaen
Single turnovers of fluorescence ATP bound to bipolar myosin filament during actinfilaments sliding.

BIOPHYSICS 9: 13-20 (2013)

 

2012年

Haruo Sugi, Takakazu Kobayashi, Teizo Tsuchiya, Shigeru Chaen,Seiryo Sugiura

Evidence for the essential role of myosin head lever arm domain and myosin
subfragment-2 in muscle contraction. In Skeletal muscle-from myogenesis to clinical relations. (ed. J. Cseri) pp.125-140. INTECHOPEN.COM (2012)

 

2012年

Haruo Sugi, Hiroki Minoda, Takuya Miyakawa, Suguru Tanokura, Shigeru Chaen, Takakazu Kobayashi

The gas environmental chamber as a powerful tool to study structural changes of living muscle thick filaments coupled with ATP hydrolysis. In Current basic and pathological approaches to the function of muscle cells and tissues-From molecules to Human (ed. H. Sugi) pp.3-26. INTECHOPEN.COM (2012)

 

2010年

Hiroyuki Okubo, Masanori Iwai, Sosuke Iwai, Shigeru Chaen.
Thermal activation energy for bidirectional movement of actin along bipolar tracks of myosin filaments.

Biochemical and Biophysical Research Communications. 396(2):539-542. (2010)

 

2010年

Hideki Shishido, Kiyoshi Nakazato, Eisaku Katayama, Shigeru Chaen and Shinsaku Maruta

Kinesin―Calmodulin fusion protein as a molecular shuttle.
J.Biochem. 147(2):213-223. (2010)

 

2008年

Haruo Sugi, Hiroki Minoda, Y Inayoshi, F Yumoto, Takuya Miyakawa, Yumiko Miyauchi, Suguru Tanokura, Tsuyoshi Akimoto, Takakazu Kobayashi, Shigeru Chaen, Seiryo Sugiura
Direct demonstration of the cross-bridge recovery stroke in muscle thick filaments in aqueous solution by using the hydration chamber.
Proceedings of National Academy of Science 105, 17396-17401 (2008)

 

2007年

Iwai, S. and Chaen, S.
Mutation in the SH1 helix reduces the activation energy of the ATP-induced conformational transition of myosin.
Biochemical and Biophysical Research Communications 357, 325-329 (2007)

 

2006年

Iwai, S., Hanamoto, D., and Chaen, S.
A point mutation in the SH1 helix alters elasticity and thermal stability of myosin II.

J. Biol. Chem. 281, 30736-30744. (2006)

 

2004年

Kobayashi, T., Saeki, Y., Chaen, S., Shirakawa, I., and Sugi, H.
Effect of deuterium oxide on contraction charasteristics and ATPase activity in
glycerinated single rabbit skeletal muscle fibers.
Biochim. Biophys. Acta 1659, 46-51. (2004)

 

2003年

Chaen, S., Yamamoto, N., Shirakawa, I., and Sugi, H.
In vitro actomyosin motility in deuterium oxide.
Adv. Exp. Med. Biol. 538, 183-192. (2003)

 

2003年

Sugi, H., and Chaen, S.,
Force-velocity relationships in actin-myosin interactions causing cytoplasmic
streaming in algal cells.
J. Exp.Biol. 206, 1971-1976. (2003)

 

2002年

Maruta, S., Mizukura, Y., and Chaen, S.,
Interaction of a new fluorescent ATP analogue with skeletal muscle myosin subfragment-1.
J. Biochem. 131, 905-911. (2002)

 

2001年

Chaen, S., Yamamoto, N., Shirakawa, I., and Sugi, H.
Effect of deuterium oxide on actomyosin motility in vitro.
Biochim. Biophys. Acta 1516, 218-223. (2001)

 

2000年

Shirakawa, I., Chaen, S., Bagshaw, C.R., and Sugi, H.
Measurement of nucleotide exchange rate constants in single rabbit soleus myofibrils
during shortening and lengthening using a fluorescent ATP analog.
Biophys. J. 78, 918-926. (2000)

 

1998年

Chaen, S., Shirakwa, I., Bagshaw, C.R., and Sugi, H.
Measurement of ATP turnover during shortening and lengthening of rabbit psoas myofibrils using a fluorescent ATP analog.
Adv. Exp. Med. Biol. 453, 569-576. (1998)

 

1998年

Sugiura, S., Kobayakawa, N., Fujita, H., Momomura, S., Chaen, S., and Sugi, H.
Distinct kinetic properties of cardiac myosin isoforms revealed by in vitro studies.
Adv. Exp. Med. Biol. 453, 125-130. (1998)

 

1998年

Watabe, S., Hirayama, Y., Nakaya, M., Kakinuma, M., Kikuchi, K., Guo, X-F., Katoh, S., Chaen, S., and Ooi, T.
Carp expresses fast skeletal myosin isoforms with altered motor functions and structural stabilities to compensate for changes in environmental temperature. J. Therm.Biol. 22, 375-390. (1998)

 

1998年

Sugi, H., Akimoto, T., Chaen, S., and Suzuki, S.
ATP-induced axial movement of myosin heads in living thick filaments recorded with a gas environmental chamber attached to the electron microscope.
Adv. Exp. Med. Biol. 453, 53-61. (1998)

 

1998年

Miyata, H., Chaen, S., Sugiura, S., and Sugi, H.
Evanescent excitation microscopy. Its application to the study of single molecular process kinetics of actomyosin motor.
Adv. Exp. Med. Biol. 453, 29-35. (1998)

 

1998年

Sugiura, S., Kobayakawa, N., Fujita, H., Yamashita, H., Momomura, S., Chaen, S., Omata, M., and Sugi, H.
Comparison of unitary displacements and forces between cardiac myosin isoforms by the optical trap technique:Molecular basis for cardiac adaptation.
Circulation Res. 82, 1029-1034. (1998)

 

1997年

Chaen, S., Shirakawa, I., Bagshaw, C.R., and Sugi, H.
Measurement of nucleotide release kinetics in single skeletal myofibrils during isometric and isovelocity contractions using fluorescent microscopy.
Biophys. J. 73, 2033-2042 (1997)

 

1997年

Sugi, H., Akimoto, T., Sutoh, K., Chaen, S., Oishi, N., and Suzuki, S.
Dynamic electron microscopy of ATP-induced myosin head movement in living muscle thick filaments.Proc. Natl. Acad. Sci. USA 94, 4378-4382. (1997)

 

1996年

Chaen, S., Nakaya, M., Guo, X-F., and Watabe, S.
Lower activation energy for sliding of F-actin on a less thermostable isoform of carp myosin.
J. Biochem. 120, 788-791. (1996)

 

1996年

Sugiuara, S., Kobayakawa, N., Momomura, S., Chaen, S., Omata, M., and Sugi, H.
Different cardiac myosin isoforms exhibit equal force-generating ability in vitro.
Biochim. Biophys. Acta 1273, 73-76. (1996)

 

1995年

Sugiuara, S., Yamashita, H., Sata, M., Momomura, S., Serizawa, T., Oiwa, K., Chaen, S., Shimmen, T., and Sugi, H.
Force-velocity relations of rat cardiac myosin isozymes sliding on algal cells actin cables in vitro.
Biochim. Biophys. Acta 1231, 69-75. (1995)

 

1995年

Chaen, S., Inoue, J., and Sugi, H.
The force-velocity relationship of the ATP-dependent actin-myosin sliding causing cytoplasmic streaming in algal cells, studied using a centrifugal microscope.
J. Exp. Biol. 198, 1021-1027 (1995)

 

1995年

Shirakwa, I., Oiwa, K., Chaen, S., Shimizu, T., Tanaka, H., and Sugi, H.
The mode of ATP-dependent microtuble-kinesin sliding in the auxotonic condition.
J. Exp. Biol. 198, 1809-1815. (1995)

 

1995年

Kobayashi, T., Gross, T., Chaen, S., Sugiura, S., Tanaka, H., and Sugi, H.
Effect of lateral forces on the movement of myosin-coated beads on actin cables studied using a centrifugal microscope.
Jpn. J. Physiol. 45, 177-186. (1995)

 

1993年

Tregear, R., Oiwa, K., Chaen, S., and Sugi, H.
Relation between magnetically-applied force and velocity in beads coated with rabbit myosin sliding on actin cables in Nitellopsis cells.
J. Muscle Res. Cell Motil. 14, 412-415. (1993)

 

1993年

Sugi, H., Oiwa, K., and Chaen, S.
Dependent of the work done by ATP-induced actin-myosin sliding on the initial baseline force: its implications for kinetic properties of myosin heads in muscle contraction.
Adv. Exp. Med. Biol. 332, 303-311. (1993)

 

1993年

Chaen, S., Oiwa, K., Kobayashi, T., Gross, T., Kamitubo, E., Shimmen, T. and Sugi, H.
Kinetic properties of the ATP-dependent actin-myosin sliding as revealed by the force-movement assay system with a centrifuge microscope.
Adv. Exp. Med. Biol. 332, 351-360. (1993)

 

1991年

Oiwa, K., Chaen, S., and Sugi, H.
Measurement of work done by ATP-induced sliding between rabbit muscle myosin and algal cell actin cables in vitro.
J. Physiol. 437, 751-763. (1991)

 

1990年

Oiwa, K., Chaen, S., Kamitsubo, E., Shimmen, T., and Sugi, H.
Steady-state force-velocity relation in the ATP-dependent sliding movement of myosin-coated beads on actin cables in vitro studied with a centrifuge microscope.
Proc. Natl. Acad. Sci. USA 87, 7893-7897. (1990)

 

1990年

Kohno, T., Chaen, S., and Shimmen, T.
Characterization of the translocator associated pollen tube organelles.
Plotoplasma 154, 179-183. (1990)

 

1989年

Chaen, S., Oiwa, K., Shimmen, T., Iwamoto, H., and Sugi, H.
Simultaneous recordings of force and sliding movement between myosin-coated glass microneedles and actin cables in vitro.
Proc. Natl. Acad. Sci. USA 86, 1510-1514. (1989)

 

1988年

Chaen, S., Shimada, M., and Sugi, H.
Cooperative interactions of myosin heads in muscle force generation.
Adv. Exp. Med. Biol. 226, 289-298. (1988)

 

1986年

Chaen, S., Shimada, M., and Sugi, H.
Evidence for cooperative interactions of myosin heads with thin filament in the force generation of vertebrate skeletal muscle fibers.
J. Biol. Chem. 261, 13632-13636. (1986)

 

1985年

Chaen, S., Shimada, M., and Sugi, H.
Fluorescence properties and contraction characteristics of ANM(N-(1-anilinonaphtyl-4)maleimide)-labeled rabbit psoas muscle fiber.
J. Biochem. 98, 939-947. (1985)

 

1984年

Gomi, S., Chaen, S., and Sugi, H.
The mode of action of maitotoxin on the membrane systems of frog skeletal muscle fibers.
Proc. Japan Acad. 60, 28-31. (1984)

 

1984年

Chaen, S. and Shimizu, H.
Substrate-concentration dependence of contraction parameters in glycerinated insect flight msucle fibers from Lethocerus derollei.
J. Biochem. 95, 839-845. (1984)

 

1982年

Chaen, S. and Sugi, H.
Substrate concentration dependence of contraction characteristics in glycerinated horseshoe crab muscle fibers.
Comp. Biochem. Physiol. 72A, 623-626. (1982)

 

1981年

Chaen, S., Kometani, K., Yamada, T., and Shimizu, H.

Substrate-concentration dependence of tension, shortening velocity and ATPase activity of glucerinated single muscle fibers.J. Biochem. 90, 1611-1621. (1981)

 

1977年

Nishiyama, K., Shimizu, H., Kometani, K., and Chaen, S.

The three state model for the elementary process of energy conversion in muscle.Biochim. Biophys. Acta 460, 523-536 (1977)

 

 

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